Hi,

I am just wondering about this Warning message (In DESeqDataSet(se, design = design, ignoreRank) :some variables in design formula are characters, converting to factors) which I never encountered in my DESeq2 use before.
My design is already a factor(male and female).

here is my dds

dds <- DESeqDataSetFromMatrix(countData = countData, colData = colData, design = ~ gender)

Thanks!

It's not a warning, just a message. There are three levels in R: message, warning, error.

gender was not a factor already, so this function fixed that for you.