Hi everyone! I am trying to design Prime Editing gRNA. As described on the software package (CRISPRseek, Version 1.27.6) page 22, I've written the following:

inputFilePath<- ("/path/to/the/file.fasta")   
pegRNA <- findgRNAs(inputFilePath, 
                       pairOutputFile = "testpairedgRNAs1.xls",
                       PAM.size = 3L,
                       gRNA.size = 20L,
                       overlap.gRNA.positions = c(17L,18L),
                       PBS.length = 15,
                       corrected.seq = "C",
                       RT.template.pattern = "D$",
                       RT.template.length = 8:30,
                       targeted.seq.length.change = 0,
                       bp.after.target.end = 15,
                       target.start = 506,
                       target.end = 506,
                       paired.orientation = "PAMin", min.gap = 20, max.gap = 90,
                       primeEditing = TRUE, 
                       findPairedgRNAOnly = TRUE,
                       )

But I get the following error message:

Error in findgRNAs(inputFilePath, pairOutputFile = "testpairedgRNAs1.xls",  : 
  unused arguments (PBS.length = 15, corrected.seq = "C", RT.template.pattern = "D$", RT.template.length = 8:30, targeted.seq.length.change = 0, bp.after.target.end = 15, target.start = 506, target.end = 506, primeEditing = TRUE)

Does anyone have any idea of what I can do to fix this problem? Am I missing something? I want also to mention that "findgRNAs" function works pretty well in finding the regular gRNAs from the same input file.

Here is my session info:

> sessionInfo()
R version 3.6.3 (2020-02-29)
Platform: x86_64-apple-darwin15.6.0 (64-bit)
Running under: macOS High Sierra 10.13.6

I'd appreciate it!

Hi Giti,

Please install R 4.0, the most recent release of Bioconductor (3.11) and CRISPRseek package 1.28.0. Functions for designing Primer Editing and Base Editing gRNAs are only available at CRISPRseek 1.28.0 or above.

For detailed information, please refer to http://bioconductor.org/packages/release/bioc/html/CRISPRseek.html.

Best regards, Julie