Reverse calculate counts using DESeq2 normalised values
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@nanuragsharma-23329
Last seen 4.8 years ago

I do not have access to suitable computational facilities to analyse raw reads of an RNAseq experiment.

Therefore I requested the raw read counts instead from the author of a paper in order to analyse the conditions myself. However the author has only provided me with the Deseq2 normalised counts. Is there a way for me to reverse calculate the raw read counts or proceed with analysis using Deseq2? If yes, which step in the vignette would I need to feed these counts into?

deseq2 rnaseq • 526 views
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@mikelove
Last seen 1 day ago
United States

Ask them again for the original counts. It’s really just easier to get the correct data than to do computational backflips to avoid getting the correct input.

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