I do not have access to suitable computational facilities to analyse raw reads of an RNAseq experiment.
Therefore I requested the raw read counts instead from the author of a paper in order to analyse the conditions myself. However the author has only provided me with the Deseq2 normalised counts. Is there a way for me to reverse calculate the raw read counts or proceed with analysis using Deseq2? If yes, which step in the vignette would I need to feed these counts into?