Use ddCT with gene specific efficiencies?
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@bettenbrock-23012
Last seen 4.7 years ago

I am using the ddCt package for evaluation of qPCR data and it works fine with the default settings. Is it possible to use gene specific efficiencies? In the vingnette it is mentioned that it is possible to use them but I have no clue where to change the default parameter thatsets equal gene efficiencies for all genes. Where can I change paramerters?

ddCt efficiency gene specific efficiencies • 724 views
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@james-w-macdonald-5106
Last seen 8 hours ago
United States

Did you read (one of) the vignettes. Seems like it's covered in sufficient detail.

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@zhang-jitao-david-5737
Last seen 4.7 years ago
Switzerland

Hi bettenbrock, as suggested by James, please have a look at the vignette and check whether your problem is solved.

If not, as the package author, I will be happy to help further. Please contact me with a minimal example in that case.

Best regards, David

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