Hi all,

I've been trying to use the MEDIPS.CpGenrich function in the MEDIPS package (with R 3.6 and BioC 3.10), which throws an error because it uses RangedData objects which are now deprecated. Has anyone either found a workaround to this or modified the MEDIPS.CpGenrich code to migrate it to GRanges?

Thanks!

Dear Emily,

Thank you for your email!

Unfortunately the MEDIPS.CpGenrich() function depends on RangedData, which is not available any more. I have not updated MEDIPS to function without RangedData and will not be able to do so in the near future. Therefore, MEDIPS.CpGenrich() is not available for the time being unless you manage to find an old R/Bioconductor setting where RangedData are still available.

I also would like to stress that we have developed a new package ‘qsea’, which has a much improved method for estimating %methylation from MeDIP-seq data. It also does not have the CG enrichment QC, but is much more flexible in terms of parameter settings etc.

Best wishes, Lukas