Entering edit mode
Fei Liu
•
0
@fei-liu-22054
Last seen 4.8 years ago
Hi all, when I use tximeta, follow the usage http://www.bioconductor.org/packages/release/bioc/vignettes/tximeta/inst/doc/tximeta ,
salmon index -t Homo_sapiens.GRCh38.cdna.all.fa.gz Homo_sapiens.GRCh38.ncrna.fa.gz -i GRCh38.index
and then
files <- file.path(dir,"quant.sf")
file.exists(files)
coldata <- data.frame(files, names="control_1", condition="A", stringsAsFactors=FALSE)
fastaFTP <- c('~/downloads/REFERENCE/Homo_sapiens.GRCh38.cdna.all.fa.gz','~/downloads/REFERENCE/Homo_sapiens.GRCh38.ncrna.fa.gz')
gtfPath <- '~/downloads/REFERENCE/Homo_sapiens.GRCh38.98.gtf.gz'
makeLinkedTxome <- function(indexDir,source,organism,release,genome,fasta,gtf,write=TRUE,jsonFile)
{indexJson <- file.path(indexDir,"info.json")
indexList <- fromJSON(indexJson)
indexSeqHash <- indexList$value0$SeqHash
index <- basename(indexDir)
std.sources <- c("Gencode", "Ensembl")
for (src in std.sources) {
if (tolower(source) == tolower(src)) {
source <- src
}
}
}
makeLinkedTxome(indexDir=indexDir,source="Ensembl",organism="Homo sapiens", release="98", genome="GRCh38", fasta=fastaFTP, gtf=gtfPath, write=FALSE)
se <- tximeta(coldata)
error:
importing quantifications
reading in files with read_tsv
1
couldn't find matching transcriptome, returning un-ranged SummarizedExperiment
I want to ask why this error occurs? Thank you all
sessionInfo()
R version 3.6.1 (2019-07-05)
Platform: x86_64-pc-linux-gnu (64-bit)
Running under: Ubuntu 18.04.1 LTS
Matrix products: default
BLAS: /usr/lib/x86_64-linux-gnu/openblas/libblas.so.3
LAPACK: /usr/lib/x86_64-linux-gnu/libopenblasp-r0.2.20.so
locale:
[1] LC_CTYPE=en_US.UTF-8 LC_NUMERIC=C
[3] LC_TIME=en_US.UTF-8 LC_COLLATE=en_US.UTF-8
[5] LC_MONETARY=en_US.UTF-8 LC_MESSAGES=en_US.UTF-8
[7] LC_PAPER=en_US.UTF-8 LC_NAME=C
[9] LC_ADDRESS=C LC_TELEPHONE=C
[11] LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C
attached base packages:
[1] stats4 parallel stats graphics grDevices utils datasets
[8] methods base
other attached packages:
[1] TxDb.Hsapiens.UCSC.hg38.knownGene_3.4.6
[2] GenomicFeatures_1.36.4
[3] AnnotationDbi_1.46.1
[4] Biobase_2.44.0
[5] GenomicRanges_1.36.1
[6] GenomeInfoDb_1.20.0
[7] IRanges_2.18.3
[8] S4Vectors_0.22.1
[9] BiocGenerics_0.30.0
[10] tximeta_1.2.2
[11] RJSONIO_1.3-1.4
loaded via a namespace (and not attached):
[1] Rcpp_1.0.3 lattice_0.20-38
[3] prettyunits_1.1.0 Rsamtools_2.0.2
[5] Biostrings_2.52.0 assertthat_0.2.1
[7] zeallot_0.1.0 digest_0.6.22
[9] BiocFileCache_1.8.0 R6_2.4.1
[11] backports_1.1.5 RSQLite_2.2.0
[13] httr_1.4.1 pillar_1.4.3
[15] zlibbioc_1.30.0 rlang_0.4.2
[17] progress_1.2.2 lazyeval_0.2.2
[19] curl_4.3 blob_1.2.0
[21] Matrix_1.2-17 BiocParallel_1.18.1
[23] readr_1.3.1 stringr_1.4.0
[25] ProtGenerics_1.16.0 RCurl_1.95-4.13
[27] bit_1.1-15.1 biomaRt_2.40.5
[29] DelayedArray_0.10.0 compiler_3.6.1
[31] rtracklayer_1.44.4 pkgconfig_2.0.3
[33] tximport_1.12.3 tidyselect_0.2.5
[35] SummarizedExperiment_1.14.1 tibble_2.1.3
[37] GenomeInfoDbData_1.2.1 matrixStats_0.55.0
[39] XML_3.98-1.20 crayon_1.3.4
[41] dplyr_0.8.3 dbplyr_1.4.2
[43] GenomicAlignments_1.20.1 bitops_1.0-6
[45] rappdirs_0.3.1 grid_3.6.1
[47] jsonlite_1.6 DBI_1.1.0
[49] AnnotationFilter_1.8.0 magrittr_1.5
[51] stringi_1.4.5 XVector_0.24.0
[53] vctrs_0.2.1 ensembldb_2.8.1
[55] tools_3.6.1 bit64_0.9-7
[57] glue_1.3.1 purrr_0.3.3
[59] hms_0.5.3 memoise_1.1.0
Thank you very much. The problem has been solved. Best regards!