How can I incorporate the Morenos-Mateo (CRISPRscan) gRNA efficiency scores into your software? I need to use T7 IVT and these scores work better in that case than Doench scores.

Thanks!

Hi Patrick,

Please download the latest dev version of CRISPRseek (>= Version: 1.27.5), which has the CRISPRscan gRNA efficacy algorithm implemented. You can download the latest version using git clone git@git.bioconductor.org:packages/CRISPRseek or wait for it to be propagated to http://bioconductor.org/packages/devel/bioc/html/CRISPRseek.html.

Please set the following parameters in offTargetAnalysis function call

rule.set <- "CRISPRscan"

featureWeightMatrixFile <- system.file("extdata", "Morenos-Mateo.csv", package = "CRISPRseek")

Please let me know if it works as expected. Thanks!

Best wishes, Julie