Total Area Sum Intensity based Normalization for label-free quantitative proteomics

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amolares • 0

@amolares-13142

Last seen 5.0 years ago

Hi!

I wonder if there is a package where Total Area Sum Intensity based Normalization is included for SWATH-MS label-free proteomics data. I would like to apply this normalization method that is usually found in MarkerView software, but I don't find it outside of there. Thank you very much in advance.

Alberto Molares Health Research Institute (IDIS) Santiago de Compostela University Hospital (CHUS) Galicia (Spain)

normalization proteomics label-free • 1.5k views

ADD COMMENT • link 5.0 years ago amolares • 0

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Could you clarify what Total Area Sum Intensity based Normalization is. It could very well be already available in MSnbase or easy to implement manually.

ADD REPLY • link 5.0 years ago Laurent Gatto 1.6k

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Really, I would like to run in R TAS (total area sum) and MLR (most likely ratio) normalizations, as showed in following paper: https://www.nature.com/articles/nmeth.2702 Many thanks

ADD REPLY • link 5.0 years ago amolares • 0

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