I am trying to finish a microarray analysis. I need to select information from the A flavus package to find deferentially expressed genes. Select is not working in Annotation Dbi. How do I create a custom select function so that R recognizes the function? Thank you. cdf=AFLAVUSa520391F (22683 affyids) annotation=aflavusa520391f
library("aflavusa520391fcdf") anno<-select(aflavusa520391fcdf, + keys=(featureNames(eset)), + columns=c("SYMBOL","GENENAME"), + keytype="PROBEID") Error in select(aflavusa520391fcdf, keys = (featureNames(eset)), columns = c("SYMBOL", : could not find function "select"
Okay, then how do I add feature data in order to find differentially expressed genes?
Your question doesn't make sense to me. I just showed you how to get the annotation data, and having the annotation data does nothing for finding differentially expressed genes. If you are asking how to put the data in the
featureData
slot, then read the help page foreSet
or perhapsExpressionSet
. If you are asking how to find differentially expressed genes, read the limma User's Guide.