CDF still not found
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ekl • 0
@ekl-22229
Last seen 5.0 years ago

I tried what you suggested about the CDF, and got the following error message.

> library(BiocManager)
Bioconductor version 3.9 (BiocManager 1.30.8), ?BiocManager::install for help
Bioconductor version '3.9' is out-of-date; the current release version '3.10'
  is available with R version '3.6'; see https://bioconductor.org/install
> install("makecdfenv")
Bioconductor version 3.9 (BiocManager 1.30.8), R 3.6.1 (2019-07-05)
Installing package(s) 'makecdfenv'
trying URL 'https://bioconductor.org/packages/3.9/bioc/bin/windows/contrib/3.6/makecdfenv_1.60.0.zip'
Content type 'application/zip' length 3419205 bytes (3.3 MB)
downloaded 3.3 MB

package ‘makecdfenv’ successfully unpacked and MD5 sums checked

The downloaded binary packages are in
        C:\Users\EKL\AppData\Local\Temp\RtmpsvwvR7\downloaded_packages
Old packages: 'BiocManager', 'boot', 'digest', 'foreign', 'hms', 'KernSmooth',
  'mgcv', 'nlme', 'rlang'
Update all/some/none? [a/s/n]: library(makecdfenv)
Update all/some/none? [a/s/n]: make.cdf.package("C:\\Users\\EKL\\Downloads\\GPL8345_AFLAVUSa520391F.cdf", "aflavusa520391fcdf", version = "0.0.1", species = "Aspergillus_flavus")
Update all/some/none? [a/s/n]: install.packages("aflavus520391fcdf", type = "source", repos = NULL)
Update all/some/none? [a/s/n]: n
> library(affy)
Loading required package: BiocGenerics
Loading required package: parallel

Attaching package: ‘BiocGenerics’

The following object is masked _by_ ‘.GlobalEnv’:

    design

The following objects are masked from ‘package:parallel’:

    clusterApply, clusterApplyLB, clusterCall, clusterEvalQ,
    clusterExport, clusterMap, parApply, parCapply, parLapply,
    parLapplyLB, parRapply, parSapply, parSapplyLB

The following objects are masked from ‘package:stats’:

    IQR, mad, sd, var, xtabs

The following objects are masked from ‘package:base’:

    anyDuplicated, append, as.data.frame, basename, cbind, colnames,
    dirname, do.call, duplicated, eval, evalq, Filter, Find, get, grep,
    grepl, intersect, is.unsorted, lapply, Map, mapply, match, mget,
    order, paste, pmax, pmax.int, pmin, pmin.int, Position, rank,
    rbind, Reduce, rownames, sapply, setdiff, sort, table, tapply,
    union, unique, unsplit, which, which.max, which.min

Loading required package: Biobase
Welcome to Bioconductor

    Vignettes contain introductory material; view with
    'browseVignettes()'. To cite Bioconductor, see
    'citation("Biobase")', and for packages 'citation("pkgname")'.

> eset<-ReadAffy(celfile.path="C:\\Users\\EKL\\Downloads\\GSE57629_RAW")
> eset
AffyBatch object
size of arrays=716x716 features (26 kb)
cdf=AFLAVUSa520391F (??? affyids)
number of samples=18
Error in getCdfInfo(object) : 
  Could not obtain CDF environment, problems encountered:
Specified environment does not contain AFLAVUSa520391F
Library - package aflavusa520391fcdf not installed
Bioconductor - aflavusa520391fcdf not available
In addition: Warning message:
missing cdf environment! in show(AffyBatch) 
CDF Package • 1.1k views
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@james-w-macdonald-5106
Last seen 1 day ago
United States

So you see this part of your code, where R is asking you a question and you just keep entering commands?

Update all/some/none? [a/s/n]: library(makecdfenv)
Update all/some/none? [a/s/n]: make.cdf.package("C:\\Users\\EKL\\Downloads\\GPL8345_AFLAVUSa520391F.cdf", "aflavusa520391fcdf", version = "0.0.1", species = "Aspergillus_flavus")
Update all/some/none? [a/s/n]: install.packages("aflavus520391fcdf", type = "source", repos = NULL)
Update all/some/none? [a/s/n]: n

While R is asking you that question nothing that you input other than the choices of a/s/n will be registered by R! So just because you tried to do something, it doesn't mean that you actually did anything.

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Also, when you are posting a question, below the box you are writing in is a preview box that shows what your post will look like. You should ensure that your formatting is correct, and you can read about how to do that here.

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Thank you so much! I am new to bioinformatics, so thank you for your patience!

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It worked! I was putting the commands in too quickly.

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