Feature intensities in affy data
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Eric Blalock ▴ 250
@eric-blalock-78
Last seen 10.2 years ago
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@rafael-a-irizarry-205
Last seen 10.2 years ago
you can get all the pms using the method pm. for example: Data <- ReadAffy() pms <- pm(Data) simliarly for mm mms <- mm(Data) the rownames of thes matrices will have the probe names followed by a number telling you what probe it is in the ID. Names <- rownames(pms) you probably dont want the numbers so use: Names <- probeNames(Data) you can then create a data.frame combining these result <- data.frame(Names,pms,mms) this last one will be slow. and use the function wrtie.table to create a text file. look at the helpfile for this function as it has many options. hope this helps, rafael On Thu, 24 Apr 2003, Eric wrote: > Hi, > > In the affy package, is there a way to get all of the feature intensities > for the entire chip output as a text file along with columns defining > probe pair #, pm/mm, and AffyID? I'm just a biologist, so type slowly :) > > -E > > At 12:04 PM 4/24/2003 +0200, you wrote: > > Send Bioconductor mailing list submissions to > bioconductor@stat.math.ethz.ch > > To subscribe or unsubscribe via the World Wide Web, visit > https://www.stat.math.ethz.ch/mailman/listinfo/bioconductor > or, via email, send a message with subject or body 'help' to > bioconductor-request@stat.math.ethz.ch > > You can reach the person managing the list at > bioconductor-owner@stat.math.ethz.ch > > When replying, please edit your Subject line so it is more > specific > than "Re: Contents of Bioconductor digest..." > > > Today's Topics: > > 1. too many cel files for RMA (J?rg Mages) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Thu, 24 Apr 2003 11:08:08 +0200 > From: J?rg Mages<mages@lrz.tu-muenchen.de> > Subject: [BioC] too many cel files for RMA > To: <bioconductor@stat.math.ethz.ch> > Message-ID: <001e01c30a41$06545d10$6db2278d@24affy> > Content-Type: text/plain > > Dear All, > > I do have a problem while reading in more than 20 cel files > using ReadAffy(). The error message is : can´t allocate > vector. Can anybody help? > > Thank´s in advance > Joerg > > [[alternate HTML version deleted]] > > > ------------------------------ > > _______________________________________________ > Bioconductor mailing list > Bioconductor@stat.math.ethz.ch > https://www.stat.math.ethz.ch/mailman/listinfo/bioconductor > > > End of Bioconductor Digest, Vol 2, Issue 26 > ******************************************* > > Eric Blalock, PhD > Dept Pharmacology, UKMC > 859 323-8033 > > STATEMENT OF CONFIDENTIALITY > > The contents of this e-mail message and any attachments are confidential > and are intended solely for addressee. The information may also be > legally privileged. This transmission is sent in trust, for the sole > purpose of delivery to the intended recipient. If you have received this > transmission in error, any use, reproduction or dissemination of this > transmission is strictly prohibited. If you are not the intended > recipient, please immediately notify the sender by reply e-mail or at > (859) 323-8033 and delete this message and its attachments, if any. >
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Crispin Miller ★ 1.1k
@crispin-miller-264
Last seen 10.2 years ago
Hi, I had a similar problem - I couldn't get pm() and mm() to work after I had uses expresso to do a mas5 normalisation on the data. I wonder if this is the trouble? Crispin > -----Original Message----- > From: Rafael A. Irizarry [mailto:ririzarr@jhsph.edu] > Sent: 24 April 2003 23:59 > To: Eric > Cc: bioconductor@stat.math.ethz.ch > Subject: Re: [BioC] Feature intensities in affy data > > > you can get all the pms using the method pm. for example: > > Data <- ReadAffy() > pms <- pm(Data) > > simliarly for mm > > mms <- mm(Data) > > the rownames of thes matrices will have the probe names followed by a > number telling you what probe it is in the ID. > > Names <- rownames(pms) > > you probably dont want the numbers so use: > > Names <- probeNames(Data) > > you can then create a data.frame combining these > > result <- data.frame(Names,pms,mms) > > this last one will be slow. > and use the function wrtie.table to create a text file. look at the > helpfile for this function as it has many options. > > hope this helps, > rafael > > > > On Thu, 24 Apr 2003, Eric wrote: > > > Hi, > > > > In the affy package, is there a way to get all of the > feature intensities > > for the entire chip output as a text file along with > columns defining > > probe pair #, pm/mm, and AffyID? I'm just a biologist, so > type slowly :) > > > > -E > > > > At 12:04 PM 4/24/2003 +0200, you wrote: > > > > Send Bioconductor mailing list submissions to > > bioconductor@stat.math.ethz.ch > > > > To subscribe or unsubscribe via the World Wide Web, visit > > > https://www.stat.math.ethz.ch/mailman/listinfo/bioconductor > > or, via email, send a message with subject or body 'help' to > > bioconductor-request@stat.math.ethz.ch > > > > You can reach the person managing the list at > > bioconductor-owner@stat.math.ethz.ch > > > > When replying, please edit your Subject line so it is more > > specific > > than "Re: Contents of Bioconductor digest..." > > > > > > Today's Topics: > > > > 1. too many cel files for RMA (J?rg Mages) > > > > > > > ---------------------------------------------------------------------- > > > > Message: 1 > > Date: Thu, 24 Apr 2003 11:08:08 +0200 > > From: J?rg Mages<mages@lrz.tu-muenchen.de> > > Subject: [BioC] too many cel files for RMA > > To: <bioconductor@stat.math.ethz.ch> > > Message-ID: <001e01c30a41$06545d10$6db2278d@24affy> > > Content-Type: text/plain > > > > Dear All, > > > > I do have a problem while reading in more than 20 cel files > > using ReadAffy(). The error message is : can?t allocate > > vector. Can anybody help? > > > > Thank?s in advance > > Joerg > > > > [[alternate HTML version deleted]] > > > > > > ------------------------------ > > > > _______________________________________________ > > Bioconductor mailing list > > Bioconductor@stat.math.ethz.ch > > https://www.stat.math.ethz.ch/mailman/listinfo/bioconductor > > > > > > End of Bioconductor Digest, Vol 2, Issue 26 > > ******************************************* > > > > Eric Blalock, PhD > > Dept Pharmacology, UKMC > > 859 323-8033 > > > > STATEMENT OF CONFIDENTIALITY > > > > The contents of this e-mail message and any attachments are > confidential > > and are intended solely for addressee. The information may also be > > legally privileged. This transmission is sent in trust, for the sole > > purpose of delivery to the intended recipient. If you have > received this > > transmission in error, any use, reproduction or > dissemination of this > > transmission is strictly prohibited. If you are not the intended > > recipient, please immediately notify the sender by reply > e-mail or at > > (859) 323-8033 and delete this message and its attachments, if any. > > > > _______________________________________________ > Bioconductor mailing list > Bioconductor@stat.math.ethz.ch > https://www.stat.math.ethz.ch/mailman/listinfo/bioconductor > -------------------------------------------------------- This email is confidential and intended solely for the use of the person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author and do not necessarily represent those of the Paterson Institute for Cancer Research or the Christie Hospital NHS Trust. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible.
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once you use expresso you have an exprSet not an AffyBatch. you have expression measures not probe level data... so no pms nor mms. On Fri, 25 Apr 2003, Crispin Miller wrote: > Hi, > I had a similar problem - I couldn't get pm() and mm() to work after I had uses expresso to do a mas5 normalisation on the data. I wonder if this is the trouble? > > Crispin > > > > > -----Original Message----- > > From: Rafael A. Irizarry [mailto:ririzarr@jhsph.edu] > > Sent: 24 April 2003 23:59 > > To: Eric > > Cc: bioconductor@stat.math.ethz.ch > > Subject: Re: [BioC] Feature intensities in affy data > > > > > > you can get all the pms using the method pm. for example: > > > > Data <- ReadAffy() > > pms <- pm(Data) > > > > simliarly for mm > > > > mms <- mm(Data) > > > > the rownames of thes matrices will have the probe names followed by a > > number telling you what probe it is in the ID. > > > > Names <- rownames(pms) > > > > you probably dont want the numbers so use: > > > > Names <- probeNames(Data) > > > > you can then create a data.frame combining these > > > > result <- data.frame(Names,pms,mms) > > > > this last one will be slow. > > and use the function wrtie.table to create a text file. look at the > > helpfile for this function as it has many options. > > > > hope this helps, > > rafael > > > > > > > > On Thu, 24 Apr 2003, Eric wrote: > > > > > Hi, > > > > > > In the affy package, is there a way to get all of the > > feature intensities > > > for the entire chip output as a text file along with > > columns defining > > > probe pair #, pm/mm, and AffyID? I'm just a biologist, so > > type slowly :) > > > > > > -E > > > > > > At 12:04 PM 4/24/2003 +0200, you wrote: > > > > > > Send Bioconductor mailing list submissions to > > > bioconductor@stat.math.ethz.ch > > > > > > To subscribe or unsubscribe via the World Wide Web, visit > > > > > https://www.stat.math.ethz.ch/mailman/listinfo/bioconductor > > > or, via email, send a message with subject or body 'help' to > > > bioconductor-request@stat.math.ethz.ch > > > > > > You can reach the person managing the list at > > > bioconductor-owner@stat.math.ethz.ch > > > > > > When replying, please edit your Subject line so it is more > > > specific > > > than "Re: Contents of Bioconductor digest..." > > > > > > > > > Today's Topics: > > > > > > 1. too many cel files for RMA (J?rg Mages) > > > > > > > > > > > ---------------------------------------------------------------------- > > > > > > Message: 1 > > > Date: Thu, 24 Apr 2003 11:08:08 +0200 > > > From: J?rg Mages<mages@lrz.tu-muenchen.de> > > > Subject: [BioC] too many cel files for RMA > > > To: <bioconductor@stat.math.ethz.ch> > > > Message-ID: <001e01c30a41$06545d10$6db2278d@24affy> > > > Content-Type: text/plain > > > > > > Dear All, > > > > > > I do have a problem while reading in more than 20 cel files > > > using ReadAffy(). The error message is : can´t allocate > > > vector. Can anybody help? > > > > > > Thank´s in advance > > > Joerg > > > > > > [[alternate HTML version deleted]] > > > > > > > > > ------------------------------ > > > > > > _______________________________________________ > > > Bioconductor mailing list > > > Bioconductor@stat.math.ethz.ch > > > https://www.stat.math.ethz.ch/mailman/listinfo/bioconductor > > > > > > > > > End of Bioconductor Digest, Vol 2, Issue 26 > > > ******************************************* > > > > > > Eric Blalock, PhD > > > Dept Pharmacology, UKMC > > > 859 323-8033 > > > > > > STATEMENT OF CONFIDENTIALITY > > > > > > The contents of this e-mail message and any attachments are > > confidential > > > and are intended solely for addressee. The information may also be > > > legally privileged. This transmission is sent in trust, for the sole > > > purpose of delivery to the intended recipient. If you have > > received this > > > transmission in error, any use, reproduction or > > dissemination of this > > > transmission is strictly prohibited. If you are not the intended > > > recipient, please immediately notify the sender by reply > > e-mail or at > > > (859) 323-8033 and delete this message and its attachments, if any. > > > > > > > _______________________________________________ > > Bioconductor mailing list > > Bioconductor@stat.math.ethz.ch > > https://www.stat.math.ethz.ch/mailman/listinfo/bioconductor > > > > -------------------------------------------------------- > > > This email is confidential and intended solely for the use of the person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author and do not necessarily represent those of the Paterson Institute for Cancer Research or the Christie Hospital NHS Trust. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. > > _______________________________________________ > Bioconductor mailing list > Bioconductor@stat.math.ethz.ch > https://www.stat.math.ethz.ch/mailman/listinfo/bioconductor >
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