Entering edit mode
Brent.OCarrigan
•
0
@brentocarrigan-21529
Last seen 5.0 years ago
Hi,
The external data for flowWorkspace
vignettes cannot seem to be loaded. No error message generated, just a blank character string returned. The external data for flowCore
loads correctly.
flowWorkspace
example
> BiocManager::install('flowWorkspace')
> library(flowWorkspace)
> flowDataPath <- system.file("extdata", package = "flowWorkspaceData")
> gs <- load_gs(file.path(flowDataPath,"gs_manual"))
Error in normalizePath(path, mustWork = TRUE) :
path[1]="/Library/Frameworks/R.framework/Versions/3.6/Resources/library/flowWorkspace/extdata/gs_manual": No such file or directory
It's true: in the flowWorkspace directory
/Library/Frameworks/R.framework/Resources/library/flowWorkspace/extdata
there is no file called gs_manual
, only empty_workspace_9.2.xml
Have tried restarting R session, re-installing flowWorkspace - no luck
flowCore
comparison:
> browseVignettes('flowCore')
> file.name <- system.file("extdata","0877408774.B08",
+ package="flowCore")
> file.name
[1] "/Library/Frameworks/R.framework/Versions/3.6/Resources/library/flowCore/extdata/0877408774.B08"
> x <- read.FCSfile.name, transformation=FALSE)
> summary(x)
FSC-H SSC-H FL1-H FL2-H FL3-H FL1-A FL4-H Time
Min. 85.0000 11.0000 0.0000 0.0000 0.0000 0.0000 0.0000 1.00
1st Qu. 385.0000 141.0000 233.0000 277.0000 90.0000 0.0000 210.0000 122.00
Median 441.0000 189.0000 545.5000 346.0000 193.0000 26.0000 279.0000 288.00
Mean 491.9644 277.9105 439.1023 366.1567 179.7122 34.0766 323.5306 294.77
3rd Qu. 518.0000 270.0000 610.0000 437.0000 264.0000 51.0000 390.0000 457.50
Max. 1023.0000 1023.0000 912.0000 1023.0000 900.0000 1023.0000 1022.0000 626.00
Session info:
> sessionInfo()
R version 3.6.0 (2019-04-26)
Platform: x86_64-apple-darwin15.6.0 (64-bit)
Running under: macOS Mojave 10.14.5
Matrix products: default
BLAS: /System/Library/Frameworks/Accelerate.framework/Versions/A/Frameworks/vecLib.framework/Versions/A/libBLAS.dylib
LAPACK: /Library/Frameworks/R.framework/Versions/3.6/Resources/lib/libRlapack.dylib
locale:
[1] en_GB.UTF-8/en_GB.UTF-8/en_GB.UTF-8/C/en_GB.UTF-8/en_GB.UTF-8
attached base packages:
[1] stats graphics grDevices utils datasets methods base
other attached packages:
[1] flowWorkspace_3.32.0 ncdfFlow_2.30.1 BH_1.69.0-1
[4] RcppArmadillo_0.9.600.4.0 flowCore_1.50.0
loaded via a namespace (and not attached):
[1] tidyselect_0.2.5 purrr_0.3.2 lattice_0.20-38 pcaPP_1.9-73 colorspace_1.4-1
[6] stats4_3.6.0 yaml_2.2.0 rlang_0.4.0 hexbin_1.27.3 pillar_1.4.2
[11] glue_1.3.1 Rgraphviz_2.28.0 BiocGenerics_0.30.0 RColorBrewer_1.1-2 matrixStats_0.54.0
[16] robustbase_0.93-5 stringr_1.4.0 zlibbioc_1.30.0 munsell_0.5.0 gtable_0.3.0
[21] mvtnorm_1.0-11 latticeExtra_0.6-28 Biobase_2.44.0 parallel_3.6.0 DEoptimR_1.0-8
[26] Rcpp_1.0.2 KernSmooth_2.23-15 corpcor_1.6.9 scales_1.0.0 BiocManager_1.30.4
[31] RcppParallel_4.4.3 graph_1.62.0 IDPmisc_1.1.19 gridExtra_2.3 digest_0.6.20
[36] stringi_1.4.3 dplyr_0.8.3 grid_3.6.0 tools_3.6.0 magrittr_1.5
[41] tibble_2.1.3 cluster_2.1.0 crayon_1.3.4 rrcov_1.4-7 pkgconfig_2.0.2
[46] MASS_7.3-51.4 flowViz_1.48.0 data.table_1.12.2 assertthat_0.2.1 rstudioapi_0.10
[51] R6_2.4.0 compiler_3.6.0
Great spot! Thanks Martin, issue solved. In the
flowWorkspace
vignettes there was not a call to specifically install the separateflowWorkspaceData
package but that has solved the issue - thank you!