I have a csv file of Ct values where the columns are the genes including reference/housekeeping genes (GAPDH, ACTB). The last column contains the class of each sample, i.e. 'control' or 'treatment'.

The rows contain the samples. The experiment was performed in duplicates, so row1 and row2 are for sample 1, row3 and row4 for sample2 and so on.

I understand that HTqPCR takes data in qPCRset format, but I am being unable to convert my csv matrix as per requirement. Please help me with stepwise instructions. It would also be great if you could help we with instructions for using NormqPCR package on the same data. Thank you so much.