Hello Heidi,

I have microRNA profiling data from 80 samples (TLDA cards A and B), and I am trying to use your package (HTqPCR) for the analysis. I have successfully combined the data from the TLDA card A and that from the TLDA B (i.e. in two separate objects) , but when I try to combine both objects with rbind, it gives an error. The code below has worked before for me, so I am not sure what is wrong now.

I would truly appreciate your help

Best

Naiara

library (HTqPCR) cat(paste(readLines(file.path(path, "NaiaraPoolA.txt"),n = 80), "\n")) cat(paste(readLines(file.path(path, "NaiaraPoolB.txt"),n = 80), "\n"))

column.info= list (flag=4, sampleName= 5, feature = 6, position = 3, Ct = 8, type=7 )

rawA <- readCtData( "NaiaraPoolA.txt",path = path, column.info=column.info,format="SDS", n.data = 80, na.value = 40) rawB <- readCtData( "NaiaraPoolB.txt",path = path, column.info=column.info,format="SDS", n.data = 80, na.value = 40) raw.bind <- rbind.qPCRset (rawA,rawB)

Error in rbind2(argl[[i]], r) : no method for coercing this S4 class to a vector