Hi limma users! I have two quick questions 1)I know that setting wt.fun=wtflags(0) in read.maimages() exclude flagged spots from normalization. But does it exclude flagged spots also from the fitting of linear model in lmFit()? 2)Is it possible that setting a different value (for example like wt.fun=wtflags(0.1) ) make p-value worse and also change the vastly the ID in the topTable() list? Thanks for any suggestion Daniela