RPM data analysis with DESeq2
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@ruhollahmb-20544
Last seen 5.4 years ago

I have a table of RPM data for gene expressions of all human genes in two groups of patients. (each group includes several samples)

I am going to use DESeq2 to explore if there is any gene whose expression significantly changes between two groups.

DESeq2, however, seems like accepts only nu-normalized counts for the RNA seq. Is there a way to feed the DESeq2 with RPM data?

deseq2 RPM gene expression • 1.9k views
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Cross-posted on Biostars: https://www.biostars.org/p/375275/

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@mikelove
Last seen 11 hours ago
United States

Not really. If you don't have access to the counts or quantification data, I would not recommend to use DESeq2.

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@ruhollahmb-20544
Last seen 5.4 years ago

thank you for clarification!

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