how can I convert a sequence to number
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@bioinformatics-10931
Last seen 2.9 years ago
United States

I am reading about a new package and I am interested in plotting logo of a protein sequence. The problem is that the author did not specify how one can convert the sequence to number

https://www.bioconductor.org/packages/devel/bioc/vignettes/motifStack/inst/doc/motifStack_HTML.html#plot-an-amino-acid-sequence-logo

Here is an example sequence

seq <- "MGLRYSIYIENPLSSPSSSYKSINDPLFHSQHRSQKNVSFITYGCRHCKTHLSSSFQIIS
RDYRGRTGTAYLMNKVVNVVEGKVEQRRMLTGDYLVCDILCHWCKRNVGWKYLQSSNDDQ
QYKEGKFILELKNICKCT"

is there anyone who could help me plot it using this package or any other package

motifStack • 813 views
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Entering edit mode
Ou, Jianhong ★ 1.3k
@ou-jianhong-4539
Last seen 4 days ago
United States

Is this a good way to show your sequence as a motif logo? It is not suggested. Whatever, you can try following code to plot your sequence.

seq <- "MGLRYSIYIENPLSSPSSSYKSINDPLFHSQHRSQKNVSFITYGCRHCKTHLSSSFQIIS
RDYRGRTGTAYLMNKVVNVVEGKVEQRRMLTGDYLVCDILCHWCKRNVGWKYLQSSNDDQ
QYKEGKFILELKNICKCT"
seq <- gsub("[^a-zA-Z]", "", seq)
seq <- strsplit(seq, "")[[1]]
rn <- sort(unique(seq))
mat <- matrix(0, nrow=length(rn), ncol=length(seq))
rownames(mat) <- rn
for(i in seq_along(seq)){mat[seq[i], i] <- 1 }
pfm <- new("pfm", mat=mat, name="your_seq_name")
plot(pfm) ##please make sure the canvas size: width >> height
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