mt.teststat
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Park, Richard ▴ 220
@park-richard-227
Last seen 10.3 years ago
Hello Everyone, I was wondering if anyone could interpret the raw p-values calculated using mt.teststat() (this function is part of the multtest package). I am using the following lines: ## To Calculate two-sided unequal variance t test values ## teststat <- mt.teststat(compSet, label, test="t", nonpara="n") rawp <- 2 * (1-pnorm(abs(teststat))) I am keeping track of the rawp values between all possible comparisons of samples. However, I have not been able to completely decipher the values that are coming out of this combination. Example: WT = 58.286, 48.842, 51.108 KO = 7.486, 6.373, 7.899 The p value calculated using "test_t.test(x[k,],xr[k,],paired=F,var.equal=F)" is 0.003219981, which is the same value that is calculated using the t.test within Excel. Mt.teststat() produces a value of 0.00000000 (basically 0, I'm not sure if it is lack of precision?). Does anyone understand why the pvalue generated from mt.teststat() is so much smaller than the traditional t.test() and what this means? Thank you, Richard Park Computational Data Analyzer Joslin Diabetes Center
multtest DECIPHER multtest DECIPHER • 2.9k views
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@wolfgang-huber-3550
Last seen 3 months ago
EMBL European Molecular Biology Laborat…
On Tue, 8 Apr 2003, Park, Richard wrote: > I was wondering if anyone could interpret the raw p-values calculated > using mt.teststat() (this function is part of the multtest package). I > am using the following lines: > > teststat <- mt.teststat(compSet, label, test="t", nonpara="n") > rawp <- 2 * (1-pnorm(abs(teststat))) Hi Richard, mt.teststat is fine. You need to use the "pt" function and not "pnorm" if you want to calculate the p-value of a t-test from the t-statistic. Also, in order to compare it to the result of ctest::t.test, there you'll have to use var.equal=TRUE. Best regards Wolfgang
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Hi Wolfgang,

 

Could you please elaborate the earlier email .

> teststat <- mt.teststat(compSet, label, test="t", nonpara="n")

> rawp <- 2 * (1-pt(abs(teststat)))

 

Do i need to include a df . 

For my study I have 12 pre and 12 post samples. (n=24)

 

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I used this for classlabel and the data frame .

I am using the function  :

Results <- mt.teststat(X,classlabel,test="wilcoxon")

 

my script 

Results <- mt.teststat(X,classlabel,test="wilcoxon")

rawp.test <- 2 * (1-pt(abs(Results),df=23))

procedures = c("Bonferroni", "Hochberg", "BH")
adjusted = mt.rawp2adjp(rawp.pnorm, procedures)
adjusted$adjp[1:10, ]

Could you please advice if the syntax is correct. I am not sure where I can put the factors you mentioned in the earlier mail above.

ctest::t.test

var.equal=TRUE.

 

Thank you

smeeta

 

 

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@smeeta-shrestha-6393
Last seen 8.9 years ago

adjusted = mt.rawp2adjp(rawp.test, procedures)

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