ChIPseeker genomicAnnotationPriority
0
1
Entering edit mode
dolata ▴ 10
@dolata-19282
Last seen 5.9 years ago

Hi, I'm trying to annotate peaks using annotatepeak (ChIPseeker) There is no problem using default genomicAnnotationPriority

peakAnno <- annotatePeak(files[[1]], tssRegion = c(-1000, 0), TxDb = txdb, level = "transcript", assignGenomicAnnotation = TRUE, genomicAnnotationPriority = c("Promoter", "5UTR", "3UTR", "Exon", "Intron", "Downstream", "Intergenic"), annoDb = "org.At.tair.db", addFlankGeneInfo = FALSE, flankDistance = 5000, sameStrand = FALSE, ignoreOverlap = FALSE, ignoreUpstream = FALSE, ignoreDownstream = FALSE, overlap = "TSS", verbose = TRUE)

However, when I move "Promoter" behind "Intron", promoters are gone from "Genomic Annotation Summary" table. I mean that promoters are not used for annotation and not that "Frequency" is 0.

Have anybody idea how to fix it?

Jakub

annotation chipseeker • 2.0k views
ADD COMMENT
0
Entering edit mode

I am meeting the same problem

ADD REPLY

Login before adding your answer.

Traffic: 703 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6