Entering edit mode
Fangxin Hong
▴
810
@fangxin-hong-912
Last seen 10.2 years ago
Dear list,
I have this 8 affy arrays under 2*2 factorial design, with duplicates
under each condition. The RNA degradation plot worries me since the
slopes
from 8 arrays are so different, with duplicates under each condition
as
one group (see the QC plots at http://cactus.salk.edu/temp/QC-1.jpeg)
I would suspect that these arrays were processed under different
levels
if amplification.
My problem is how to handle this data set beside doing the
normalization?
Will this pattern seriously bias the result? I read some previous
message
about this topic, just hope to get more information.
Many thanks!
Fangxin
--------------------
Fangxin Hong Ph.D.
Plant Biology Laboratory
The Salk Institute
10010 N. Torrey Pines Rd.
La Jolla, CA 92037
E-mail: fhong at salk.edu
(Phone): 858-453-4100 ext 1105
--------------------
Fangxin Hong Ph.D.
Plant Biology Laboratory
The Salk Institute
10010 N. Torrey Pines Rd.
La Jolla, CA 92037
E-mail: fhong at salk.edu
(Phone): 858-453-4100 ext 1105