Filtering probesets for HuEx ST1.0 after probeset normalization
0
0
Entering edit mode
@genomic_region-13050
Last seen 3.8 years ago

Hi all,  

I'm working with exon level microarray analysis. I've two annotation files - probeset and transcripts. I normalize data on two targets - gene and probesets to get estimates at gene level and exon level respectively.   

After filtering genes that aren't in probeset annotation and probesets for which genes aren't available I end up with 17K genes and 580K exon probesets.   

Is there a way I can filter these 580K probesets? How?      

I cannot find any function in oligo to calculate dabg.   

Second, can I only work with probesets that are main? filtering probes in affymetrix data   

I have 228349 probesets that are main.   

I maybe wrong in using correct terminology (exon, exon probsets), please correct and pardon me.   

 

Thanks!!

microarray exons probefiltering • 754 views
ADD COMMENT

Login before adding your answer.

Traffic: 695 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6