Hello,

I am wondering what the procedure would be to create a flowframe from a .csv file that was not obtained from a flow cytometry machine? The specific example we were thinking of was measuring single-cell gene expression. We have written a script that does some basic dimensionality reduction and outputs the data in different visual formats and works fine with .fcs files, but because we built it to work around .fcs files we are having troubles using .csv files.

The simplest alternative (at least to the best of our admittedly limited knowledge), apart from generalizing the whole script, would be to convert the .csv into a .fcs file and then work with it from there. The expression data is easy enough to work out, but how would you go about creating the annotated data frame specifically?

Thank you.

-Timothy