I am running flowAI on some flow cytometry data with ~250k events, however I keep getting this error:
Error: vector memory exhausted (limit reached?)
I've looked it up and it's likely something that has changed since R 3.5 with the introduction of the R_MAX_VSIZE environment variable, but when I set R_MAX_VSIZE to something huge (200Gb) before running R freezes and needs a restart. My data isn't long enough to cause this error (26Kb), so it must be an issue with the QC report generated.
Update:
I've rerun flow_auto_qc() suppressing the generation of the html QC report and ran into the same error. I'm at a loss for whats causing this.
Any suggestions are appreciated since I'd like to use this tool in the near future.
> sessionInfo()
R version 3.5.1 (2018-07-02)
Platform: x86_64-apple-darwin15.6.0 (64-bit)
Running under: macOS High Sierra 10.13.5
Matrix products: default
BLAS: /System/Library/Frameworks/Accelerate.framework/Versions/A/Frameworks/vecLib.framework/Versions/A/libBLAS.dylib
LAPACK: /Library/Frameworks/R.framework/Versions/3.5/Resources/lib/libRlapack.dylib
locale:
[1] en_US.UTF-8/en_US.UTF-8/en_US.UTF-8/C/en_US.UTF-8/en_US.UTF-8
attached base packages:
[1] stats graphics grDevices utils datasets methods base
other attached packages:
[1] flowAI_1.10.1
loaded via a namespace (and not attached):
[1] pcaPP_1.9-73 Rcpp_0.12.18 DEoptimR_1.0-8 compiler_3.5.1 pillar_1.3.0
[6] RColorBrewer_1.1-2 plyr_1.8.4 bindr_0.1.1 tools_3.5.1 digest_0.6.15
[11] evaluate_0.11 tibble_1.4.2 gtable_0.2.0 lattice_0.20-35 pkgconfig_2.0.1
[16] rlang_0.2.1 graph_1.58.0 yaml_2.2.0 parallel_3.5.1 mvtnorm_1.0-8
[21] bindrcpp_0.2.2 stringr_1.3.1 knitr_1.20 dplyr_0.7.6 cluster_2.0.7-1
[26] rprojroot_1.3-2 tidyselect_0.2.4 stats4_3.5.1 grid_3.5.1 robustbase_0.93-2
[31] glue_1.3.0 Biobase_2.40.0 rrcov_1.4-4 R6_2.2.2 rmarkdown_1.10
[36] reshape2_1.4.3 purrr_0.2.5 ggplot2_3.0.0 corpcor_1.6.9 magrittr_1.5
[41] backports_1.1.2 htmltools_0.3.6 scales_0.5.0 matrixStats_0.54.0 BiocGenerics_0.26.0
[46] changepoint_2.2.2 assertthat_0.2.0 flowCore_1.46.1 colorspace_1.3-2 labeling_0.3
[51] stringi_1.2.4 lazyeval_0.2.1 munsell_0.5.0 crayon_1.3.4 zoo_1.8-3