A question about log_transformed
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liushang • 0
@liushang-16396
Last seen 5.2 years ago

I would like you to tell me why the filtered expression matrix M should log-transformed after adding a pseudo-count of 1: M’ = log2(M + 1).

Thank you!!

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@james-w-macdonald-5106
Last seen 2 hours ago
United States

Adding a pseudo-count prior to taking logs protects against taking logs of zero. In addition, if you have lots of really small counts it can also help reduce the variability inherent in having small counts, particularly if you use a larger prior count.

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