Hello,
I ran DEXSeq on a linux based cluster and saved the DEXSeqResults object as a .csv file to run locally for further analysis and visualization. When I read in the .csv to R, I am able to see it appear properly in the Data Environment, but none of the commands that should run from this work. I have updated bioconductor and all packages along with my version of R.
Example:
> mcols(dxr1)$description
Error in (function (classes, fdef, mtable) :
unable to find an inherited method for function ‘mcols’ for signature ‘"data.frame"’
or
> DEXSeqHTML(object=dxr)
Error in DEXSeqHTML(object = dxr) :
is(object, "DEXSeqResults") is not TRUE
I need to run the main part of DEXSeq on the cluster due to memory issues, but would like to be able to analyze and visualize locally. Any help would be appreciated.
Best,
Christina
Hi Christina, can you post the complete code that you are using along with the output of your sessionInfo()?
The most common and recommended way to save R objects, move them to a different computer and open them there again are the
saveandloadfunctions in R. It looks like that the CSV file you saved from the cluster only contains part of theDEXSeqResultsobject, and what you loaded is not aDEXSeqResultsobject but just adata.frame, for which what you are trying to do (DEXSeqHTML) cannot work.