CAGEr: consensusCluster doesn't have dominant CTSS info
0
1
Entering edit mode
Jingwen ▴ 10
@jingwen-15978
Last seen 6.5 years ago
Sweden/Stockholm

Hi,

I am using the latest CAGEr (1.22.1) in R 3.5.0

When following the demo in CAGEr website, I found the consensusClusterGR function didn't return dominant CTSS information when specifying sample name:

> consensusClustersGR( ce, sample = "zf_unfertilized_egg", returnInterquantileWidth = TRUE, qLow = 0.1, qUp = 0.9)

The output is exactly the same as the output in the demo webpage, no dominant CTSS. Furthermore, when exporting consensusCluster to bed file:

> exportToBed(object = ce, what = "consensusClusters", qLow = 0.1, qUp = 0.9,oneFile=FALSE)

I got such error message: Error in consensusClustersQuantileLow(object) : Not supported for ‘CAGEexp’ objects. Use ‘consensusClustersQuantile()’ instead.

Then I tried with the original CAGEr format, CAGEset. There was still no dominant ctss info when calling consensusClusterGR function. Again error messge when exporting consensusCluster to bed files.

> exportToBed(object = ce, what = "consensusClusters", qLow = 0.1, qUp = 0.9, oneFile = FALSE)
Error in dominant_ctss_start[clusters.q$dominant_ctss == clusters.q$start] <- clusters.q$start[clusters.q$dominant_ctss ==  : 
  NAs are not allowed in subscripted assignments

Did I miss some critical step before exporting consensus clusters? Thanks for your help.

 

Best,
Jingwen

cager • 1.0k views
ADD COMMENT
0
Entering edit mode

Thanks Jingwen, I will correct this. By the way, I am also tracking this issue on GitHub: https://github.com/charles-plessy/CAGEr/issues/1. If you would like to submit a patch you are very welcome !

ADD REPLY

Login before adding your answer.

Traffic: 629 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6