Hi,

I got somewhat lost in the parameters of RIPseeker. I would like to do peak calling on the data produced with TruSeq Smalll RNA Illumina protocol. It is a stranded library prep protocol -  sequencing reads from read 1 map to the antisense strand and those from read 2 map to the sense strand.

Should I use

reverseComplement = TRUE or FALSE

strandType = "+", "-", or "*"

Thank you!