mva functions
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Jason Skelton ▴ 510
@jason-skelton-135
Last seen 10.2 years ago
I'm trying to use the mva functions dist & hclust however after a while I get the following message: TypVSNdist <- dist(typVSN, method = "euclidean", diag = FALSE, upper = FALSE) Error: cannot allocate vector of size 589776 Kb > typVSNcluster <- hclust(dist(typVSN@h)) Error: cannot allocate vector of size 589776 Kb I'm running R in linux, any help much appreciated regards Jason
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Laurent Gautier ★ 2.3k
@laurent-gautier-29
Last seen 10.2 years ago
Dear Jason, We would need to know a bit more about your settings, like what one get by doing 'dim(typVSN)' or 'object.size(typVSN)', and also the amount of RAM your machine has.... Hopin' it helps, Laurent On Thu, Mar 20, 2003 at 06:16:38PM +0000, Jason Skelton wrote: > > > I'm trying to use the mva functions dist & hclust > however after a while I get the following message: > > TypVSNdist <- dist(typVSN, method = "euclidean", diag = FALSE, upper = > FALSE) > Error: cannot allocate vector of size 589776 Kb > > > typVSNcluster <- hclust(dist(typVSN@h)) > Error: cannot allocate vector of size 589776 Kb > > I'm running R in linux, any help much appreciated > > regards > > Jason > > _______________________________________________ > Bioconductor mailing list > Bioconductor@stat.math.ethz.ch > https://www.stat.math.ethz.ch/mailman/listinfo/bioconductor -- -------------------------------------------------------------- currently at the National Yang-Ming University in Taipei, Taiwan -------------------------------------------------------------- Laurent Gautier CBS, Building 208, DTU PhD. Student DK-2800 Lyngby,Denmark tel: +45 45 25 24 89 http://www.cbs.dtu.dk/laurent
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Hi The files I'm trying to run through hclust etc have the following sizes are these sizes "normal" for the output from vsn ? MalVSN and typVSN are four slide experiments each with 12288 spot features YeastVSN is from one slide with 12288 features > object.size(MalVSN) [1] 984976 > object.size(typVSN) [1] 984976 > object.size(YeastVSN1) [1] 381904 each one returns the following message Error: cannot allocate vector of size 589776 Kb Unfortunately I'm not sure of the specifcations of the machine I'm running it from (Dec Alpha) and my systems administration group aren't forthcoming apologies if this doesn't help much Thanks for everyones help Jason On Fri, 21 Mar 2003, Laurent Gautier wrote: >and also the amount of RAM your machine has....> > > TypVSNdist <- dist(typVSN, method = "euclidean", diag = FALSE, upper = > > FALSE) > > Error: cannot allocate vector of size 589776 Kb > > > typVSNcluster <- hclust(dist(typVSN@h))
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On Mon, 24 Mar 2003, Jason Skelton wrote: > The files I'm trying to run through hclust etc have the following sizes Hi Jason, You probably want to reduce the number of genes from 12488 to something like a few hundred before clustering. With 10^4 genes the distance matrix (which needs to be calculated as an intermediate step by hclust) has size of 10^8, which corresponds to roughly 1 Gigabyte. I would not expect that all 12488 rows of your data carry relevant information, esp. if you have only 4 conditions. Also, it could be hard to display the cluster carpet or heatmap with more than a few hundred rows since your display may not have that many pixels. Best regards Wolfgang
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@wolfgang-huber-3550
Last seen 11 weeks ago
EMBL European Molecular Biology Laborat…
Hi Jason, How big is your data matrix? Best regards Wolfgang Huber On Thu, 20 Mar 2003, Jason Skelton wrote: > > I'm trying to use the mva functions dist & hclust > however after a while I get the following message: > > TypVSNdist <- dist(typVSN, method = "euclidean", diag = FALSE, upper = > FALSE) > Error: cannot allocate vector of size 589776 Kb > > > typVSNcluster <- hclust(dist(typVSN@h)) > Error: cannot allocate vector of size 589776 Kb > > I'm running R in linux, any help much appreciated > > regards >
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