Affy Mouse Annotation
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Entering edit mode
trhood • 0
@trhood-15406
Last seen 6.7 years ago

Hello!

I have a question about associating probeIDs from an Affy GeoDataset GSE85507, I downloaded the series matrix and got the normalized data file with 17000+ probes and 283 samples. I tried to associate the ID_REF numbers (example "6747308") using the moex10stprobeset.db, the dataset says they used "[MoEx-1_0-st] Affymetrix Mouse Exon 1.0 ST Array [transcript (gene) version]". I keep getting an error message that says the probe ids are not valid for that database. Are these numbers not actually probe ids? How do I know how to associate them to the mgi_symbol? 

 

My code: 

library(dplyr)
library(AnnotationDbi)
library(moex10stprobeset.db)

#set working directory
setwd(".../GeoDownloads/GSE85507")

#load the data

e <- read.csv("data.csv", stringsAsFactors = FALSE)
clin <- read.csv("clin.csv", stringsAsFactors = FALSE)

#get probeIDs
ids <- e$ID_REF

# map the ids
mapped_ids <- mapIds(moex10stprobeset.db, keys=as.character(ids), c("SYMBOL"), keytype="PROBEID")

 

########

Error message:

Error in .testForValidKeys(x, keys, keytype, fks) : 
  None of the keys entered are valid keys for 'PROBEID'. Please use the keys method to see a listing of valid arguments.

annotation affymetrix • 1.1k views
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Entering edit mode
@james-w-macdonald-5106
Last seen 10 hours ago
United States

As you have already noted, the data you have are the transcript level probesets, so you need the moex10sttranscriptcluster.db package, not the probeset version.

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