Hello!
I have a question about associating probeIDs from an Affy GeoDataset GSE85507, I downloaded the series matrix and got the normalized data file with 17000+ probes and 283 samples. I tried to associate the ID_REF numbers (example "6747308") using the moex10stprobeset.db, the dataset says they used "[MoEx-1_0-st] Affymetrix Mouse Exon 1.0 ST Array [transcript (gene) version]". I keep getting an error message that says the probe ids are not valid for that database. Are these numbers not actually probe ids? How do I know how to associate them to the mgi_symbol?
My code:
library(dplyr)
library(AnnotationDbi)
library(moex10stprobeset.db)
#set working directory
setwd(".../GeoDownloads/GSE85507")
#load the data
e <- read.csv("data.csv", stringsAsFactors = FALSE)
clin <- read.csv("clin.csv", stringsAsFactors = FALSE)
#get probeIDs
ids <- e$ID_REF
# map the ids
mapped_ids <- mapIds(moex10stprobeset.db, keys=as.character(ids), c("SYMBOL"), keytype="PROBEID")
########
Error message:
Error in .testForValidKeys(x, keys, keytype, fks) :
None of the keys entered are valid keys for 'PROBEID'. Please use the keys method to see a listing of valid arguments.