genbankr fails when reading GBK file
1
0
Entering edit mode
@monther-alhamdoosh-10001
Last seen 5.4 years ago
Australia/Melbourne/CSL Limited

Hi Gabriel, 

I was trying to use your package to read a Genbank file but it failed with the following error: 

> gb = readGenBank(gbFile, verbose = TRUE)
Error in validObject(.Object) : 
  invalid class “GRanges” object: 'mcols(x)' is not parallel to 'x'
> traceback()
10: stop(msg, ": ", errors, domain = NA)
9: validObject(.Object)
8: initialize(value, ...)
7: initialize(value, ...)
6: new(Class, seqnames = seqnames, ranges = ranges, strand = strand, 
       elementMetadata = mcols, seqinfo = seqinfo)
5: new_GRanges("GRanges", seqnames = seqnames, ranges = ranges, 
       strand = strand, mcols = mcols, seqlengths = seqlengths, 
       seqinfo = seqinfo)
4: GRanges(seqnames = stk$seqnames, ranges = IRanges(start = stk$start, 
       end = stk$end), strand = stk$strand)
3: fill_stack_df(resthang$FEATURES[typs == "source"])
2: parseGenBank(text = text, partial = partial, verbose = verbose, 
       ret.seq = TRUE)
1: readGenBank(gbFile, verbose = TRUE)
> sessionInfo()
R version 3.4.2 (2017-09-28)
Platform: x86_64-apple-darwin15.6.0 (64-bit)
Running under: macOS Sierra 10.12.6

Matrix products: default
BLAS: /System/Library/Frameworks/Accelerate.framework/Versions/A/Frameworks/vecLib.framework/Versions/A/libBLAS.dylib
LAPACK: /Library/Frameworks/R.framework/Versions/3.4/Resources/lib/libRlapack.dylib

locale:
[1] en_AU.UTF-8/en_AU.UTF-8/en_AU.UTF-8/C/en_AU.UTF-8/en_AU.UTF-8

attached base packages:
[1] stats     graphics  grDevices utils     datasets  methods   base     

other attached packages:
[1] genbankr_1.6.0       BiocInstaller_1.28.0

loaded via a namespace (and not attached):
 [1] Rcpp_0.12.14               compiler_3.4.2             GenomeInfoDb_1.14.0       
 [4] XVector_0.18.0             GenomicFeatures_1.30.0     prettyunits_1.0.2         
 [7] bitops_1.0-6               tools_3.4.2                zlibbioc_1.24.0           
[10] progress_1.1.2             biomaRt_2.34.0             digest_0.6.12             
[13] bit_1.1-12                 BSgenome_1.46.0            lattice_0.20-35           
[16] RSQLite_2.0                memoise_1.1.0              tibble_1.3.4              
[19] rlang_0.1.4                Matrix_1.2-12              DelayedArray_0.4.1        
[22] DBI_0.7                    parallel_3.4.2             GenomeInfoDbData_0.99.1   
[25] rtracklayer_1.38.2         stringr_1.2.0              knitr_1.17                
[28] Biostrings_2.46.0          S4Vectors_0.16.0           IRanges_2.12.0            
[31] grid_3.4.2                 stats4_3.4.2               bit64_0.9-7               
[34] Biobase_2.38.0             R6_2.2.2                   AnnotationDbi_1.40.0      
[37] XML_3.98-1.9               RMySQL_0.10.13             BiocParallel_1.12.0       
[40] blob_1.1.0                 magrittr_1.5               matrixStats_0.52.2        
[43] GenomicAlignments_1.14.1   Rsamtools_1.30.0           BiocGenerics_0.24.0       
[46] GenomicRanges_1.30.0       SummarizedExperiment_1.8.0 assertthat_0.2.0          
[49] stringi_1.1.6              RCurl_1.95-4.8             VariantAnnotation_1.24.2  
> 

Do you know what would be the reason for this error? 

Thanks,

Monther

genbankr • 1.2k views
ADD COMMENT
0
Entering edit mode
@beckergabriel-9990
Last seen 6.4 years ago

Monther,

Apologies for not responding sooner. I'm not familiar with this error. I haven't seen it before. Can you provide me the gbk file in question so I can do some debugging?

Thanks,

~G

ADD COMMENT

Login before adding your answer.

Traffic: 881 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6