Hi! I am trying to do phenograph analysis on a small set of hand-gated flow cytometry data. Under the GUI, I selected files, markers, etc..  I am getting the following error:

Extract expression data...
Error in (function (..., deparse.level = 1)  : 
  number of columns of matrices must match (see arg 3)

I was once able to do the analysis with the same set of files (all events) earlier. Not sure what might be wrong now. Please help. 

Thanks,

Monali