R2.2 Bioconductor 1.7 Windows XP Hi all, I was happily using the kooperberg function for background correction until I upgraded to 1.7. Using the same input (cut and pasted from the same document to avoid typos), I get an error in 1.7 that I don't get when using 1.6 and R2.1.1. Here's the relevant input/output: > targetsB <- readTargets("targetsB.txt") > spottypes <- readSpotTypes() #I define a function called filters here, but have left it out for brevity > RGB <- read.maimages(targetsB$FileName, source="genepix", wt.fun=filters) Read B115_R480_G430.gpr Read B116_R480_G430.gpr Read B117_R480_G430.gpr Read B118_R480_G430.gpr Read B119_R480_G430.gpr Read B120_R480_G430.gpr Read B121_R480_G430.gpr Read B122_R480_G430.gpr Read B123_R480_G430.gpr Read B124_R480_G430.gpr Read B125_R480_G430.gpr Read B126_R480_G430.gpr > RGBgenes <- readGAL("MOB-1-3.anno.gal") > RGBlayout <- getLayout(RGBgenes) > read.series(targetsB$FileName, suffix=NULL, skip=31, sep="\t", quote="\"") [1] "12 slides read" > RGBmodel <- kooperberg(targetsB$FileName, layout=RGBlayout) Error in kooperberg(targetsB$FileName, layout = RGBlayout) : Data missing from RG$other: re-run read.maimages with other=c("F635 SD","B635 SD","F532 SD","B532 SD","B532 Mean","B635 Mean","F Pixels","B Pixels", "Block") In addition: Warning message: Coercing LHS to a list Any ideas? Thanks, Matt --------------------------------------------- College of Agriculture and Life Sciences Web Mail. http://ag.arizona.edu