Hello,
I'm attempting to use refseq genes for plotting with Gviz and I am having trouble creating a txdb from UCSC to begin the process, but I'm getting an error:
> library(GenomicFeatures)
> hg19.refseq.db<-makeTxDbFromUCSC(genome="hg19", table="refGene")
Download the refGene table ... Error: Bad Request
> sessionInfo()
R version 3.4.3 (2017-11-30)
Platform: x86_64-w64-mingw32/x64 (64-bit)
Running under: Windows >= 8 x64 (build 9200)
Matrix products: default
locale:
[1] LC_COLLATE=English_United States.1252 LC_CTYPE=English_United States.1252 LC_MONETARY=English_United States.1252
[4] LC_NUMERIC=C LC_TIME=English_United States.1252
attached base packages:
[1] stats4 parallel stats graphics grDevices utils datasets methods base
other attached packages:
[1] GenomicFeatures_1.30.0 AnnotationDbi_1.40.0 Biobase_2.38.0 GenomicRanges_1.30.1 GenomeInfoDb_1.14.0
[6] IRanges_2.12.0 S4Vectors_0.16.0 BiocGenerics_0.24.0 biomaRt_2.34.1
loaded via a namespace (and not attached):
[1] Rcpp_0.12.14 pillar_1.0.1 compiler_3.4.3 XVector_0.18.0
[5] prettyunits_1.0.2 bitops_1.0-6 tools_3.4.3 progress_1.1.2
[9] zlibbioc_1.24.0 digest_0.6.13 bit_1.1-12 RSQLite_2.0
[13] memoise_1.1.0 tibble_1.4.1 lattice_0.20-35 rlang_0.1.6
[17] Matrix_1.2-12 DelayedArray_0.4.1 DBI_0.7 yaml_2.1.16
[21] GenomeInfoDbData_1.0.0 rtracklayer_1.38.2 stringr_1.2.0 httr_1.3.1
[25] Biostrings_2.46.0 bit64_0.9-7 grid_3.4.3 R6_2.2.2
[29] BiocParallel_1.12.0 RMySQL_0.10.13 XML_3.98-1.9 blob_1.1.0
[33] magrittr_1.5 matrixStats_0.52.2 GenomicAlignments_1.14.1 Rsamtools_1.30.0
[37] SummarizedExperiment_1.8.1 assertthat_0.2.0 stringi_1.1.6 RCurl_1.95-4.10
Thanks,
James Dalgleish
I still can't seem to quite download it yet using install.packages() and the rtracklayer page (https://bioconductor.org/packages/release/bioc/html/rtracklayer.html) doesn't reflect the new version. Let me know if there is a way to access it otherwise.
Is there a working github repo I can use to install it with devtools::install.github()?
You should wait for the new version to appear.