a problem with Metadat package: hgu133a2
2
0
Entering edit mode
Shi, Tao ▴ 720
@shi-tao-199
Last seen 9.5 years ago
Hi Chenwei, I ran into this problem totally by random. The annotation for this particular probeset is diffrent from what I got from NetAffx: > version _ platform i386-pc-mingw32 arch i386 os mingw32 system i386, mingw32 status major 2 minor 1.1 year 2005 month 06 day 20 language R > get("221770_at", hgu133a2GENENAME) [1] "solute carrier family 4, sodium bicarbonate transporter-like, member 10" which should be for "206830_at". > get("206830_at", hgu133a2GENENAME) [1] "solute carrier family 4, sodium bicarbonate transporter-like, member 10" The annotation I got from NetAffx is: 221770_at ribulose-5-phosphate-3-epimerase Could you please check what's going on? Thanks, ...Tao
Annotation Annotation • 1.1k views
ADD COMMENT
0
Entering edit mode
@ting-yuan-liu-fhcrc-1221
Last seen 10.3 years ago
Hi Tao, ChenWei is not the current maintainer for the meta data packages, but I will try to figure what's wrong here for him. Could you provide the result of sessionInfo()? People can get more information about the versions of the packages you are using and then will be easier to determine the source of problem you have. Also, could you tell me which file did you get from NetAffx? When was this file release? Affymetrix released their annotation files at least twice after our last release on May. If you are comparing the package of BioC 1.6 with the file you recently downloaded, you might find that there are some inconsistent because Affymetrix updated the file. Any way, please provide the information I mentioned above so that I can determine if you were in such a inconsistent situation. Best, Ting-Yuan On Tue, 4 Oct 2005, Shi, Tao wrote: > Hi Chenwei, > > I ran into this problem totally by random. The annotation for this particular probeset is > diffrent from what I got from NetAffx: > > > version > _ > platform i386-pc-mingw32 > arch i386 > os mingw32 > system i386, mingw32 > status > major 2 > minor 1.1 > year 2005 > month 06 > day 20 > language R > > get("221770_at", hgu133a2GENENAME) > [1] "solute carrier family 4, sodium bicarbonate transporter-like, member 10" > > which should be for "206830_at". > > > get("206830_at", hgu133a2GENENAME) > [1] "solute carrier family 4, sodium bicarbonate transporter-like, member 10" > > > The annotation I got from NetAffx is: > > 221770_at ribulose-5-phosphate-3-epimerase > > > Could you please check what's going on? Thanks, > > ...Tao > > _______________________________________________ > Bioconductor mailing list > Bioconductor at stat.math.ethz.ch > https://stat.ethz.ch/mailman/listinfo/bioconductor >
ADD COMMENT
0
Entering edit mode
Hi Ting-Yuan, Sorry for my forgetfulness. I was thinking about providing the package version information before I sent the email out, but still forgot it. Anyway, here are the information you need: > sessionInfo() R version 2.1.1, 2005-06-20, i386-pc-mingw32 attached base packages: [1] "splines" "tools" "methods" "stats" "graphics" "grDevices" "utils" [8] "datasets" "base" other attached packages: hgu133plus2 KEGG hgu95av2 GOstats multtest genefilter survival "1.8.4" "1.8.1" "1.8.4" "1.1.3" "1.6.0" "1.6.3" "2.18" xtable RBGL annotate graph Biobase Ruuid cluster "1.2-5" "1.3.13" "1.5.16" "1.5.9" "1.5.12" "1.5.3" "1.10.1" GO TSP hu6800 hgu133a2 "1.8.2" "0.1" "1.8.4" "1.8.4" I got the NetAffx annotation from the NetAffx analysis center where you can input your probe set ID and get the annotation interactively. I've also checked the affy annotation file ("HG-U133A_2_annot.csv") I downloaded on 6/20/05 from this website (http://www.affymetrix.com/support/technical/byproduct.affx?product=hg u133-20). It has the same annotation as shown on the NetAffx website now. I was also suspecting that Affy changed their annotations after the package was released, until I checked another hgu133a2 annotation file I have ("HG-U133A_2 gene info.xls", which I downloaded from dChip website on 2/2004). It's annotation is also consistent with the current NetAffx anno. ...Tao --- Ting-Yuan Liu <tliu at="" fhcrc.org=""> wrote: > > Hi Tao, > > ChenWei is not the current maintainer for the meta data packages, but I > will try to figure what's wrong here for him. > > Could you provide the result of sessionInfo()? People can get more > information about the versions of the packages you are using and then will > be easier to determine the source of problem you have. > > Also, could you tell me which file did you get from NetAffx? When was > this file release? Affymetrix released their annotation files at least > twice after our last release on May. If you are comparing the package of > BioC 1.6 with the file you recently downloaded, you might find that there > are some inconsistent because Affymetrix updated the file. > > Any way, please provide the information I mentioned above so that I can > determine if you were in such a inconsistent situation. > > Best, > Ting-Yuan > > On Tue, 4 Oct 2005, Shi, Tao wrote: > > > Hi Chenwei, > > > > I ran into this problem totally by random. The annotation for this particular probeset is > > diffrent from what I got from NetAffx: > > > > > version > > _ > > platform i386-pc-mingw32 > > arch i386 > > os mingw32 > > system i386, mingw32 > > status > > major 2 > > minor 1.1 > > year 2005 > > month 06 > > day 20 > > language R > > > get("221770_at", hgu133a2GENENAME) > > [1] "solute carrier family 4, sodium bicarbonate transporter-like, member 10" > > > > which should be for "206830_at". > > > > > get("206830_at", hgu133a2GENENAME) > > [1] "solute carrier family 4, sodium bicarbonate transporter-like, member 10" > > > > > > The annotation I got from NetAffx is: > > > > 221770_at ribulose-5-phosphate-3-epimerase > > > > > > Could you please check what's going on? Thanks, > > > > ...Tao > > > > _______________________________________________ > > Bioconductor mailing list > > Bioconductor at stat.math.ethz.ch > > https://stat.ethz.ch/mailman/listinfo/bioconductor > > >
ADD REPLY
0
Entering edit mode
John Zhang ★ 2.9k
@john-zhang-6
Last seen 10.3 years ago
221770_at is mapped to BE964473 by the chip manufacture and thus to Hs.333958 according to UniGene. BioC builds the annotation packages base on NCBI and does not guarantee that the annotation will agree with that from other sources. It is totally up to you whether to trust NCBI or NetAffx. >X-Original-To: jzhang at jimmy.harvard.edu >Delivered-To: jzhang at jimmy.harvard.edu >DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; s=s1024; d=yahoo.com; h=Message-ID:Received:Date:From:Subject:To:Cc:In-Reply-To:MIME- Version:Content-T ype:Content-Transfer-Encoding; b=MHVPEHM+OGZuRyQIH/S7QAVSX7LpLy9jD3934Q9H/QW8tvzoIyhHrx8EIfLssadFoM6N HdNtUFC28q bRWMWZTjsjWK1XjQrn3wnxR4wlqNuPR3+VfEXujY4Fg36mU/t198aJ0csBkraChJgrxkjW Yp2+dBy/GY 8GK2/Gx8mw3J0= ; >Date: Tue, 4 Oct 2005 16:39:50 -0700 (PDT) >From: "Shi, Tao" <shidaxia at="" yahoo.com=""> >To: Ting-Yuan Liu <tliu at="" fhcrc.org=""> >MIME-Version: 1.0 >Received-SPF: pass hypatia.math.ethz.ch: 129.132.145.15 is authenticated by a trusted mechanism) >Received-SPF: none (hypatia: domain of shidaxia at yahoo.com does not designate permitted sender hosts) >X-Virus-Scanned: by amavisd-new at stat.math.ethz.ch >Cc: bioconductor at stat.math.ethz.ch >Subject: Re: [BioC] a problem with Metadat package: hgu133a2 >X-BeenThere: bioconductor at stat.math.ethz.ch >X-Mailman-Version: 2.1.6 >List-Id: The Bioconductor Project Mailing List <bioconductor.stat.math.ethz.ch> >List-Unsubscribe: <https: stat.ethz.ch="" mailman="" listinfo="" bioconductor="">, <mailto:bioconductor-request at="" stat.math.ethz.ch?subject="unsubscribe"> >List-Archive: <https: stat.ethz.ch="" pipermail="" bioconductor=""> >List-Post: <mailto:bioconductor at="" stat.math.ethz.ch=""> >List-Help: <mailto:bioconductor-request at="" stat.math.ethz.ch?subject="help"> >List-Subscribe: <https: stat.ethz.ch="" mailman="" listinfo="" bioconductor="">, <mailto:bioconductor-request at="" stat.math.ethz.ch?subject="subscribe"> >Content-Transfer-Encoding: 7bit >X-Spam-Checker-Version: SpamAssassin 3.0.1 (2004-10-22) on pascal.dfci.harvard.edu >X-Spam-Level: >X-Spam-Status: No, score=-2.2 required=3.0 tests=AWL,BAYES_00 autolearn=ham version=3.0.1 > >Hi Ting-Yuan, > >Sorry for my forgetfulness. I was thinking about providing the package version information before >I sent the email out, but still forgot it. > >Anyway, here are the information you need: > >> sessionInfo() >R version 2.1.1, 2005-06-20, i386-pc-mingw32 > >attached base packages: >[1] "splines" "tools" "methods" "stats" "graphics" "grDevices" "utils" >[8] "datasets" "base" > >other attached packages: >hgu133plus2 KEGG hgu95av2 GOstats multtest genefilter survival > "1.8.4" "1.8.1" "1.8.4" "1.1.3" "1.6.0" "1.6.3" "2.18" > xtable RBGL annotate graph Biobase Ruuid cluster > "1.2-5" "1.3.13" "1.5.16" "1.5.9" "1.5.12" "1.5.3" "1.10.1" > GO TSP hu6800 hgu133a2 > "1.8.2" "0.1" "1.8.4" "1.8.4" > > >I got the NetAffx annotation from the NetAffx analysis center where you can input your probe set >ID and get the annotation interactively. I've also checked the affy annotation file >("HG-U133A_2_annot.csv") I downloaded on 6/20/05 from this website >(http://www.affymetrix.com/support/technical/byproduct.affx?product=h gu133-20). It has the same >annotation as shown on the NetAffx website now. I was also suspecting that Affy changed their >annotations after the package was released, until I checked another hgu133a2 annotation file I >have ("HG-U133A_2 gene info.xls", which I downloaded from dChip website on 2/2004). It's >annotation is also consistent with the current NetAffx anno. > >...Tao > > > >--- Ting-Yuan Liu <tliu at="" fhcrc.org=""> wrote: > >> >> Hi Tao, >> >> ChenWei is not the current maintainer for the meta data packages, but I >> will try to figure what's wrong here for him. >> >> Could you provide the result of sessionInfo()? People can get more >> information about the versions of the packages you are using and then will >> be easier to determine the source of problem you have. >> >> Also, could you tell me which file did you get from NetAffx? When was >> this file release? Affymetrix released their annotation files at least >> twice after our last release on May. If you are comparing the package of >> BioC 1.6 with the file you recently downloaded, you might find that there >> are some inconsistent because Affymetrix updated the file. >> >> Any way, please provide the information I mentioned above so that I can >> determine if you were in such a inconsistent situation. >> >> Best, >> Ting-Yuan >> >> On Tue, 4 Oct 2005, Shi, Tao wrote: >> >> > Hi Chenwei, >> > >> > I ran into this problem totally by random. The annotation for this particular probeset is >> > diffrent from what I got from NetAffx: >> > >> > > version >> > _ >> > platform i386-pc-mingw32 >> > arch i386 >> > os mingw32 >> > system i386, mingw32 >> > status >> > major 2 >> > minor 1.1 >> > year 2005 >> > month 06 >> > day 20 >> > language R >> > > get("221770_at", hgu133a2GENENAME) >> > [1] "solute carrier family 4, sodium bicarbonate transporter- like, member 10" >> > >> > which should be for "206830_at". >> > >> > > get("206830_at", hgu133a2GENENAME) >> > [1] "solute carrier family 4, sodium bicarbonate transporter- like, member 10" >> > >> > >> > The annotation I got from NetAffx is: >> > >> > 221770_at ribulose-5-phosphate-3-epimerase >> > >> > >> > Could you please check what's going on? Thanks, >> > >> > ...Tao >> > >> > _______________________________________________ >> > Bioconductor mailing list >> > Bioconductor at stat.math.ethz.ch >> > https://stat.ethz.ch/mailman/listinfo/bioconductor >> > >> > >_______________________________________________ >Bioconductor mailing list >Bioconductor at stat.math.ethz.ch >https://stat.ethz.ch/mailman/listinfo/bioconductor Jianhua Zhang Department of Medical Oncology Dana-Farber Cancer Institute 44 Binney Street Boston, MA 02115-6084
ADD COMMENT
0
Entering edit mode
Hi John, Thank you very much for clearfying it. I see your point. But I really have no idea why NCBI maps BE964473 to Hs. 333958, since all the top hits from BLASTing the 221770_at target sequence or BE964473 itself have annotation of 'ribulose-5-phosphate-3-epimerase', which should belong to UniGene cluster Hs.282260. One interesting thing is that BE964473 and the most of sequences in Hs.282260 and some other sequences used to belong to a same currently-retired UniGene cluster, Hs.125845. I guess NetAffx won this one........ best, ...Tao --- John Zhang <jzhang at="" jimmy.harvard.edu=""> wrote: > 221770_at is mapped to BE964473 by the chip manufacture and thus to Hs.333958 > according to UniGene. BioC builds the annotation packages base on NCBI and does > not guarantee that the annotation will agree with that from other sources. It is > totally up to you whether to trust NCBI or NetAffx. > > > > >X-Original-To: jzhang at jimmy.harvard.edu > >Delivered-To: jzhang at jimmy.harvard.edu > >DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; s=s1024; d=yahoo.com; > h=Message-ID:Received:Date:From:Subject:To:Cc:In-Reply-To:MIME- Version:Content-T > ype:Content-Transfer-Encoding; > b=MHVPEHM+OGZuRyQIH/S7QAVSX7LpLy9jD3934Q9H/QW8tvzoIyhHrx8EIfLssadFoM 6NHdNtUFC28q > bRWMWZTjsjWK1XjQrn3wnxR4wlqNuPR3+VfEXujY4Fg36mU/t198aJ0csBkraChJgrxk jWYp2+dBy/GY > 8GK2/Gx8mw3J0= ; > >Date: Tue, 4 Oct 2005 16:39:50 -0700 (PDT) > >From: "Shi, Tao" <shidaxia at="" yahoo.com=""> > >To: Ting-Yuan Liu <tliu at="" fhcrc.org=""> > >MIME-Version: 1.0 > >Received-SPF: pass hypatia.math.ethz.ch: 129.132.145.15 is authenticated by a > trusted mechanism) > >Received-SPF: none (hypatia: domain of shidaxia at yahoo.com does not designate > permitted sender hosts) > >X-Virus-Scanned: by amavisd-new at stat.math.ethz.ch > >Cc: bioconductor at stat.math.ethz.ch > >Subject: Re: [BioC] a problem with Metadat package: hgu133a2 > >X-BeenThere: bioconductor at stat.math.ethz.ch > >X-Mailman-Version: 2.1.6 > >List-Id: The Bioconductor Project Mailing List <bioconductor.stat.math.ethz.ch> > >List-Unsubscribe: <https: stat.ethz.ch="" mailman="" listinfo="" bioconductor="">, > <mailto:bioconductor-request at="" stat.math.ethz.ch?subject="unsubscribe"> > >List-Archive: <https: stat.ethz.ch="" pipermail="" bioconductor=""> > >List-Post: <mailto:bioconductor at="" stat.math.ethz.ch=""> > >List-Help: <mailto:bioconductor-request at="" stat.math.ethz.ch?subject="help"> > >List-Subscribe: <https: stat.ethz.ch="" mailman="" listinfo="" bioconductor="">, > <mailto:bioconductor-request at="" stat.math.ethz.ch?subject="subscribe"> > >Content-Transfer-Encoding: 7bit > >X-Spam-Checker-Version: SpamAssassin 3.0.1 (2004-10-22) on > pascal.dfci.harvard.edu > >X-Spam-Level: > >X-Spam-Status: No, score=-2.2 required=3.0 tests=AWL,BAYES_00 autolearn=ham > version=3.0.1 > > > >Hi Ting-Yuan, > > > >Sorry for my forgetfulness. I was thinking about providing the package version > information before > >I sent the email out, but still forgot it. > > > >Anyway, here are the information you need: > > > >> sessionInfo() > >R version 2.1.1, 2005-06-20, i386-pc-mingw32 > > > >attached base packages: > >[1] "splines" "tools" "methods" "stats" "graphics" "grDevices" > "utils" > >[8] "datasets" "base" > > > >other attached packages: > >hgu133plus2 KEGG hgu95av2 GOstats multtest genefilter > survival > > "1.8.4" "1.8.1" "1.8.4" "1.1.3" "1.6.0" "1.6.3" > "2.18" > > xtable RBGL annotate graph Biobase Ruuid > cluster > > "1.2-5" "1.3.13" "1.5.16" "1.5.9" "1.5.12" "1.5.3" > "1.10.1" > > GO TSP hu6800 hgu133a2 > > "1.8.2" "0.1" "1.8.4" "1.8.4" > > > > > >I got the NetAffx annotation from the NetAffx analysis center where you can > input your probe set > >ID and get the annotation interactively. I've also checked the affy annotation > file > >("HG-U133A_2_annot.csv") I downloaded on 6/20/05 from this website > >(http://www.affymetrix.com/support/technical/byproduct.affx?product =hgu133-20). > It has the same > >annotation as shown on the NetAffx website now. I was also suspecting that > Affy changed their > >annotations after the package was released, until I checked another hgu133a2 > annotation file I > >have ("HG-U133A_2 gene info.xls", which I downloaded from dChip website on > 2/2004). It's > >annotation is also consistent with the current NetAffx anno. > > > >...Tao > > > > > > > >--- Ting-Yuan Liu <tliu at="" fhcrc.org=""> wrote: > > > >> > >> Hi Tao, > >> > >> ChenWei is not the current maintainer for the meta data packages, but I > >> will try to figure what's wrong here for him. > >> > >> Could you provide the result of sessionInfo()? People can get more > >> information about the versions of the packages you are using and then will > >> be easier to determine the source of problem you have. > >> > >> Also, could you tell me which file did you get from NetAffx? When was > >> this file release? Affymetrix released their annotation files at least > >> twice after our last release on May. If you are comparing the package of > >> BioC 1.6 with the file you recently downloaded, you might find that there > >> are some inconsistent because Affymetrix updated the file. > >> > >> Any way, please provide the information I mentioned above so that I can > >> determine if you were in such a inconsistent situation. > >> > >> Best, > >> Ting-Yuan > >> > >> On Tue, 4 Oct 2005, Shi, Tao wrote: > >> > >> > Hi Chenwei, > >> > > >> > I ran into this problem totally by random. The annotation for this > particular probeset is > >> > diffrent from what I got from NetAffx: > >> > > >> > > version > >> > _ > >> > platform i386-pc-mingw32 > >> > arch i386 > >> > os mingw32 > >> > system i386, mingw32 > >> > status > >> > major 2 > >> > minor 1.1 > >> > year 2005 > >> > month 06 > >> > day 20 > >> > language R > >> > > get("221770_at", hgu133a2GENENAME) > >> > [1] "solute carrier family 4, sodium bicarbonate transporter- like, member > 10" > >> > > >> > which should be for "206830_at". > >> > > >> > > get("206830_at", hgu133a2GENENAME) > >> > [1] "solute carrier family 4, sodium bicarbonate transporter- like, member > 10" > >> > > >> > > >> > The annotation I got from NetAffx is: > >> > > >> > 221770_at ribulose-5-phosphate-3-epimerase > >> > > >> > > >> > Could you please check what's going on? Thanks, > >> > > >> > ...Tao > >> > > >> > _______________________________________________ > >> > Bioconductor mailing list > >> > Bioconductor at stat.math.ethz.ch > >> > https://stat.ethz.ch/mailman/listinfo/bioconductor > >> > > >> > > > >_______________________________________________ > >Bioconductor mailing list > >Bioconductor at stat.math.ethz.ch > >https://stat.ethz.ch/mailman/listinfo/bioconductor > > Jianhua Zhang > Department of Medical Oncology > Dana-Farber Cancer Institute > 44 Binney Street > Boston, MA 02115-6084 > >
ADD REPLY
0
Entering edit mode
Hi Tao, Please report your observation to NCBI. After NCBI fixes this bug, our packages will be updated while we use their new information. Thanks for this observation. Best, Ting-Yuan On Wed, 5 Oct 2005, Shi, Tao wrote: > Hi John, > > Thank you very much for clearfying it. I see your point. > > But I really have no idea why NCBI maps BE964473 to Hs. 333958, since all the top hits from > BLASTing the 221770_at target sequence or BE964473 itself have annotation of > 'ribulose-5-phosphate-3-epimerase', which should belong to UniGene cluster Hs.282260. One > interesting thing is that BE964473 and the most of sequences in Hs.282260 and some other sequences > used to belong to a same currently-retired UniGene cluster, Hs.125845. > > I guess NetAffx won this one........ > > best, > > ...Tao > > > --- John Zhang <jzhang at="" jimmy.harvard.edu=""> wrote: > > > 221770_at is mapped to BE964473 by the chip manufacture and thus to Hs.333958 > > according to UniGene. BioC builds the annotation packages base on NCBI and does > > not guarantee that the annotation will agree with that from other sources. It is > > totally up to you whether to trust NCBI or NetAffx. > > > > > > > > >X-Original-To: jzhang at jimmy.harvard.edu > > >Delivered-To: jzhang at jimmy.harvard.edu > > >DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; s=s1024; d=yahoo.com; > > h=Message-ID:Received:Date:From:Subject:To:Cc:In-Reply-To:MIME- Version:Content-T > > ype:Content-Transfer-Encoding; > > b=MHVPEHM+OGZuRyQIH/S7QAVSX7LpLy9jD3934Q9H/QW8tvzoIyhHrx8EIfLssadF oM6NHdNtUFC28q > > bRWMWZTjsjWK1XjQrn3wnxR4wlqNuPR3+VfEXujY4Fg36mU/t198aJ0csBkraChJgr xkjWYp2+dBy/GY > > 8GK2/Gx8mw3J0= ; > > >Date: Tue, 4 Oct 2005 16:39:50 -0700 (PDT) > > >From: "Shi, Tao" <shidaxia at="" yahoo.com=""> > > >To: Ting-Yuan Liu <tliu at="" fhcrc.org=""> > > >MIME-Version: 1.0 > > >Received-SPF: pass hypatia.math.ethz.ch: 129.132.145.15 is authenticated by a > > trusted mechanism) > > >Received-SPF: none (hypatia: domain of shidaxia at yahoo.com does not designate > > permitted sender hosts) > > >X-Virus-Scanned: by amavisd-new at stat.math.ethz.ch > > >Cc: bioconductor at stat.math.ethz.ch > > >Subject: Re: [BioC] a problem with Metadat package: hgu133a2 > > >X-BeenThere: bioconductor at stat.math.ethz.ch > > >X-Mailman-Version: 2.1.6 > > >List-Id: The Bioconductor Project Mailing List <bioconductor.stat.math.ethz.ch> > > >List-Unsubscribe: <https: stat.ethz.ch="" mailman="" listinfo="" bioconductor="">, > > <mailto:bioconductor-request at="" stat.math.ethz.ch?subject="unsubscribe"> > > >List-Archive: <https: stat.ethz.ch="" pipermail="" bioconductor=""> > > >List-Post: <mailto:bioconductor at="" stat.math.ethz.ch=""> > > >List-Help: <mailto:bioconductor-request at="" stat.math.ethz.ch?subject="help"> > > >List-Subscribe: <https: stat.ethz.ch="" mailman="" listinfo="" bioconductor="">, > > <mailto:bioconductor-request at="" stat.math.ethz.ch?subject="subscribe"> > > >Content-Transfer-Encoding: 7bit > > >X-Spam-Checker-Version: SpamAssassin 3.0.1 (2004-10-22) on > > pascal.dfci.harvard.edu > > >X-Spam-Level: > > >X-Spam-Status: No, score=-2.2 required=3.0 tests=AWL,BAYES_00 autolearn=ham > > version=3.0.1 > > > > > >Hi Ting-Yuan, > > > > > >Sorry for my forgetfulness. I was thinking about providing the package version > > information before > > >I sent the email out, but still forgot it. > > > > > >Anyway, here are the information you need: > > > > > >> sessionInfo() > > >R version 2.1.1, 2005-06-20, i386-pc-mingw32 > > > > > >attached base packages: > > >[1] "splines" "tools" "methods" "stats" "graphics" "grDevices" > > "utils" > > >[8] "datasets" "base" > > > > > >other attached packages: > > >hgu133plus2 KEGG hgu95av2 GOstats multtest genefilter > > survival > > > "1.8.4" "1.8.1" "1.8.4" "1.1.3" "1.6.0" "1.6.3" > > "2.18" > > > xtable RBGL annotate graph Biobase Ruuid > > cluster > > > "1.2-5" "1.3.13" "1.5.16" "1.5.9" "1.5.12" "1.5.3" > > "1.10.1" > > > GO TSP hu6800 hgu133a2 > > > "1.8.2" "0.1" "1.8.4" "1.8.4" > > > > > > > > >I got the NetAffx annotation from the NetAffx analysis center where you can > > input your probe set > > >ID and get the annotation interactively. I've also checked the affy annotation > > file > > >("HG-U133A_2_annot.csv") I downloaded on 6/20/05 from this website > > >(http://www.affymetrix.com/support/technical/byproduct.affx?produ ct=hgu133-20). > > It has the same > > >annotation as shown on the NetAffx website now. I was also suspecting that > > Affy changed their > > >annotations after the package was released, until I checked another hgu133a2 > > annotation file I > > >have ("HG-U133A_2 gene info.xls", which I downloaded from dChip website on > > 2/2004). It's > > >annotation is also consistent with the current NetAffx anno. > > > > > >...Tao > > > > > > > > > > > >--- Ting-Yuan Liu <tliu at="" fhcrc.org=""> wrote: > > > > > >> > > >> Hi Tao, > > >> > > >> ChenWei is not the current maintainer for the meta data packages, but I > > >> will try to figure what's wrong here for him. > > >> > > >> Could you provide the result of sessionInfo()? People can get more > > >> information about the versions of the packages you are using and then will > > >> be easier to determine the source of problem you have. > > >> > > >> Also, could you tell me which file did you get from NetAffx? When was > > >> this file release? Affymetrix released their annotation files at least > > >> twice after our last release on May. If you are comparing the package of > > >> BioC 1.6 with the file you recently downloaded, you might find that there > > >> are some inconsistent because Affymetrix updated the file. > > >> > > >> Any way, please provide the information I mentioned above so that I can > > >> determine if you were in such a inconsistent situation. > > >> > > >> Best, > > >> Ting-Yuan > > >> > > >> On Tue, 4 Oct 2005, Shi, Tao wrote: > > >> > > >> > Hi Chenwei, > > >> > > > >> > I ran into this problem totally by random. The annotation for this > > particular probeset is > > >> > diffrent from what I got from NetAffx: > > >> > > > >> > > version > > >> > _ > > >> > platform i386-pc-mingw32 > > >> > arch i386 > > >> > os mingw32 > > >> > system i386, mingw32 > > >> > status > > >> > major 2 > > >> > minor 1.1 > > >> > year 2005 > > >> > month 06 > > >> > day 20 > > >> > language R > > >> > > get("221770_at", hgu133a2GENENAME) > > >> > [1] "solute carrier family 4, sodium bicarbonate transporter- like, member > > 10" > > >> > > > >> > which should be for "206830_at". > > >> > > > >> > > get("206830_at", hgu133a2GENENAME) > > >> > [1] "solute carrier family 4, sodium bicarbonate transporter- like, member > > 10" > > >> > > > >> > > > >> > The annotation I got from NetAffx is: > > >> > > > >> > 221770_at ribulose-5-phosphate-3-epimerase > > >> > > > >> > > > >> > Could you please check what's going on? Thanks, > > >> > > > >> > ...Tao > > >> > > > >> > _______________________________________________ > > >> > Bioconductor mailing list > > >> > Bioconductor at stat.math.ethz.ch > > >> > https://stat.ethz.ch/mailman/listinfo/bioconductor > > >> > > > >> > > > > > >_______________________________________________ > > >Bioconductor mailing list > > >Bioconductor at stat.math.ethz.ch > > >https://stat.ethz.ch/mailman/listinfo/bioconductor > > > > Jianhua Zhang > > Department of Medical Oncology > > Dana-Farber Cancer Institute > > 44 Binney Street > > Boston, MA 02115-6084 > > > > > > _______________________________________________ > Bioconductor mailing list > Bioconductor at stat.math.ethz.ch > https://stat.ethz.ch/mailman/listinfo/bioconductor >
ADD REPLY

Login before adding your answer.

Traffic: 538 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6