Keith,

GUIDEseq package can be used to analyze CIRCLE-seq too. During the preprocessing steps, you will not need to remove adaptors introduced in the GUIDE-seq protocol. In addition, you will need to set the max.R1.len =150 and max.R2.len = 150 using the GUIDEseqAnalysis function in GUIDEseq package assuming your sequence run produces 150bp paired-end reads.

Best regards,

Julie