Sylvain FORET wrote: > TEXTORIS Julien wrote: > >> Hi, >> >> Could someone send me an imagene file as an example ? >> I tried to write a read function for my quantifier software (BZSCAN), >> and i try to follow the read.imagene function as i also have two >> separate files. But as i don't know the imagene format, i don't >> understand all the code of the function, and what i have to change. >> >> Thanks for your help, >> >> Julien > > > What you want to use is read.maimage, not read.imagene, unless your > software's output is exactly similar to that of Imagene. > > Let's assume that your software outputs comma separated files with at > least 4 columns: > the red channel foreground column called "Cy5F" > the red channel background column called "Cy5B" > the green channel foreground column called "Cy3F" > the green channel background column called "Cy3B". > > You just have to do something like that: > > >targets <- readTargets('yourTargets') > >RG <- read.maimages(targets$FileName, > columns=list(Rf="Cy5F", > Gf="Cy3F", > Rb="Cy5B", > Gb="Cy3B"), > sep=",") > > Of course you can have more column in your datafile and read them to > use them (eg.) for weighting. > > Hope it helps, > > SF > Thanks, Sylvain, i knew that option, but my data are in two separate files, for complex hybridation(sample), whih i call R, and vector hybridation, chich i call G. In fact i could write a script to first parse the two files and create a temporary file that i would read like that. But i think it would be easier just to type : read.maimage(files, source="bzscan"). And for that, i simply have to rewrite the read.imagene function into read.bzscan. Don't you agree ? Julien