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rbronste
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@rbronste-12189
Last seen 5.0 years ago
A little confused about this, wanted too filter out lines in the matrix with read counts lower than 10 to speed up DESeq2 processing using the following:
dds <- dds[ rowSums(counts(dds)) > 10, ]
However just as before the filtering, after the filtering countData looks like this:
head(countData) MBV1 MBV2 MBV3 FBV1 FBV2 FBV3 MBE7 MBE8 MBE9 FBE1 FBE2 FBE3 1 1 1 1 1 1 1 1 66 1 1 50 34 2 11 1 1 1 1 1 6 98 1 11 100 1 3 1 1 1 1 1 1 1 1 1 116 108 1 4 1 1 22 2 84 1 1 4 1 64 1 40 5 1 1 18 74 74 1 102 1 126 22 1 1 6 1 1 1 1 44 1 1 1 122 1 1 1
Wondering where I am going wrong? Thank you.
Thank you! Stupid mistake on my part for sure...