Question

How many genes are appropriate for GO enrichment analysis

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Jack ▴ 20

@jack-14069

Last seen 5.2 years ago

Hi all,

I got a list of differential expressed genes about 4000. I want to do the GO enrichment, can I use all the DE genes?

What is the appropriate number of genes to do GO enrichment analysis?

go annotate rna-seq clusterprofiler • 2.7k views

ADD COMMENT • link updated 7.3 years ago by k.vitting.seerup ▴ 120 • written 7.3 years ago by Jack ▴ 20

score 0 · Answer 1 · 2017-10-18

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k.vitting.seerup ▴ 120

@kvittingseerup-7956

Last seen 17 months ago

European Union

Yes you can use all DE genes (probably nice to seperate into up and down). There is no upper limit for gene-set analysis.

ADD COMMENT • link 7.3 years ago k.vitting.seerup ▴ 120